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Thyroglobulin antibody

Thyroglobulin antibody validated for immunohistochemistry on 76 different Normal Tissues

Product Specifications

Product Name Alternative

AITD3, hTG, TDH3, Tg, Tgn

UniProt

P01266

Reactivity

Human

Clonality

Monoclonal

Clone

MSVA-189R

Conjugation

Unconjugated

Dilution

1:100-1:200

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Thyroid: A strong staining is seen in follicular cells and the colloid. The stroma is typically also stained due to a contamination artifact. Negative Control: Colon: All cells must not show any staining. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Tested Applications

IHC

Host or Source

Rabbit

Isotype

IgG

Available Sizes

Frequently Asked Questions

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