CD45RA antibody
CD45RA antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
UniProt
P08575
Reactivity
Human
Immunogen
Stimulated human leukocytes
Clonality
Monoclonal
Clone
MSVA-472M
Conjugation
Unconjugated
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Tonsil: A strong membranous CD45RA immunostaining should be seen in the majority of lymphocytes. Negative Control: Tonsil: CD45RA immunostaining should be absent in epithelial cells. Cellular Localization: Cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2a, kappa
Available Sizes
Frequently Asked Questions
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