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One-step TUNEL Flow Cytometry Apoptosis Kit (Green, FITC)

Product Specifications

Detection Method

Fluorometric method; Flow cytometry

Assay Principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with flow cytometry.

Sample Type

Cell samples

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Citation 01

https://www.sciencedirect.com/science/article/pii/S1385894724085279

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1111/acel.14391

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1002/VIW.20230056

Citation 04

https://www.sciencedirect.com/science/article/pii/S0944711325000765

Citation 05

https://iovs.arvojournals.org/article.aspx?articleid=2793780

Citation 06

https://www.sciencedirect.com/science/article/pii/S0009279724000991

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10968670/

Citation 08

https://www.nature.com/articles/s42003-023-05169-3

Citation 09

https://www.sciencedirect.com/science/article/pii/S0898656823000827

Citation 10

https://www.sciencedirect.com/science/article/pii/S0093691X2400431X

Detection Instrument

Flow Cytometer

Available Sizes

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