Caspase 3/7 Activity Assay Kit (Colorimetric Method)

Product Specifications

Detection Method

Colorimetric method

Assay Principle

Caspase 3, also known as CPP32, Yama or apopain, belongs to the CED-3 subfamily of the caspase family. It is a key enzyme in the process of apoptosis and the most studied caspase in mammalian cells. Caspase 3 exists in the cytoplasm in the form of zymogen under normal condition and has no activity. In the stage of apoptosis, caspase 3 is activated, and the activated caspase 3 consists of two large subunits and two small subunits, which cleaves the corresponding cytoplasmic and nuclear substrates and ultimately leads to apoptosis. This caspase 3/7 activity assay kit is based on caspase 3/7 that can catalyze the substrate Ac-DEVD-pNA to generate yellow pNA (p-Nitroaniline) . The pNA has a strong absorption near 405 nm, The activity of caspase 3/7 can be calculated by determining the absorbance at 405 nm.

Sample Type

Cell and tissue samples

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Citation 01

https://pubs.acs.org/doi/abs/10.1021/acs.est.4c10984

Citation 02

https://www.tandfonline.com/doi/abs/10.2147/IJN.S417928

Citation 03

https://www.sciencedirect.com/science/article/pii/S0925521423003162

Citation 04

https://www.sciencedirect.com/science/article/pii/S0925521423001369

Citation 05

https://www.sciencedirect.com/science/article/pii/S0141813025014394

Citation 06

https://www.sciencedirect.com/science/article/pii/S0141813025006841

Citation 07

https://www.sciencedirect.com/science/article/pii/S0141813024082667

Citation 08

https://www.sciencedirect.com/science/article/pii/S0141813025035822

Citation 09

https://www.sciencedirect.com/science/article/pii/S0141813025042424

Citation 10

https://www.mdpi.com/2073-4409/12/23/2734

Citation 11

https://www.mdpi.com/2072-6643/14/21/4605

Citation 12

https://www.sciencedirect.com/science/article/pii/S0925521422002678

Detection Instrument

Spectrophotometer; Microplate reader

Available Sizes

Curated Selection

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