TUNEL In Situ Apoptosis Kit (HRP-DAB Method)

Product Specifications

Detection Method

Colorimetric method

Assay Principle

When cells undergo apoptosis, endonuclease enzymes are activated to cleave the genomic DNA between nucleosomes. When genomic DNA is cleaved, the exposed 3'-OH can be added with a biotinylated dUTP (Biotin-dUTP) due to the catalysis of Terminal Deoxynucleotidyl Transferase (TdT), followed by the binding of horseradish Peroxidase-labeled Streptavidin (Streptavidin-HRP) . Thereafter, through the HRP catalysis, the apoptotic cells are marked by DAB, which can be detected by ordinary microscopy. This is the principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) for detecting apoptosis.

Sample Type

Paraffin section; frozen section; cell slide

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Citation 01

https://link.springer.com/article/10.1186/s12943-024-02074-z

Citation 02

https://www.sciencedirect.com/science/article/pii/S2452199X25001860

Citation 03

https://advanced.onlinelibrary.wiley.com/doi/abs/10.1002/adfm.202420344

Citation 04

https://www.sciencedirect.com/science/article/pii/S2452199X25001781

Citation 05

https://pubs.acs.org/doi/abs/10.1021/acsnano.4c07085

Citation 06

https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202407493

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202401731

Citation 08

https://advanced.onlinelibrary.wiley.com/doi/abs/10.1002/advs.202414468

Citation 09

https://www.sciencedirect.com/science/article/pii/S1385894724061448

Citation 10

https://www.sciencedirect.com/science/article/pii/S2213231724001058

Citation 11

https://aacrjournals.org/cancerres/article/doi/10.1158/0008-5472.CAN-23-1796/733406

Citation 12

https://www.sciencedirect.com/science/article/pii/S2213231723001842

Detection Instrument

Light microscope

Available Sizes

Curated Selection

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