One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 555)

Product Specifications

Detection Method

Fluorometric method

Assay Principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

Sample Type

Paraffin section; frozen section; cell slide

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Citation 01

https://www.science.org/doi/abs/10.1126/sciimmunol.adc9081

Citation 02

https://aacrjournals.org/cancerres/article/doi/10.1158/0008-5472.CAN-23-2491/735128

Citation 03

https://www.nature.com/articles/s41401-023-01218-z

Citation 04

https://biosignaling.biomedcentral.com/articles/10.1186/s12964-024-01479-z

Citation 05

https://www.ahajournals.org/doi/abs/10.1161/ATVBAHA.123.319119

Citation 06

https://www.nature.com/articles/s41420-023-01740-3

Citation 07

https://www.sciencedirect.com/science/article/pii/S0891584924011298

Citation 08

https://onlinelibrary.wiley.com/doi/abs/10.1002/glia.24449

Citation 09

https://pmc.ncbi.nlm.nih.gov/articles/PMC12147254/

Citation 10

https://journals.physiology.org/doi/abs/10.1152/ajpcell.00348.2023

Citation 11

https://www.mdpi.com/1422-0067/24/13/11210

Citation 12

https://www.sciencedirect.com/science/article/pii/S1567576924000158

Detection Instrument

Fluorescence Microscope

Available Sizes

Curated Selection

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