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One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 647)

Product Specifications

Detection Method

Fluorometric method

Assay Principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

Sample Type

Paraffin section; frozen section; cell slide

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Citation 01

https://link.springer.com/article/10.1007/s00401-023-02675-w

Citation 02

https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-024-02780-2

Citation 03

https://www.cell.com/developmental-cell/abstract/S1534-5807(25)00002-4

Citation 04

https://www.embopress.org/doi/abs/10.1038/s44321-025-00233-3

Citation 05

https://www.embopress.org/doi/abs/10.1038/s44319-024-00109-6

Citation 06

https://www.sciencedirect.com/science/article/pii/S0944711323006487

Citation 07

https://academic.oup.com/stmcls/advance-article-abstract/doi/10.1093/stmcls/sxad037/7174085

Citation 08

https://www.mdpi.com/2073-4409/13/13/1074

Citation 09

https://www.cell.com/iscience/fulltext/S2589-0042(24)02165-5

Citation 10

https://www.hindawi.com/journals/jir/2022/4589182/

Citation 11

https://link.springer.com/article/10.1007/s12602-025-10516-3

Citation 12

https://www.jstage.jst.go.jp/article/jts/47/9/47_349/_article/-char/ja/

Detection Instrument

Laser confocal microscope (or Fluorescence microscope with Cy5 filter set)

Available Sizes

Frequently Asked Questions

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