One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488)

Product Specifications

Detection Method

Fluorometric method

Assay Principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

Sample Type

Paraffin section; frozen section; cell slide

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Citation 01

https://www.cell.com/cell/pdf/S0092-8674(23)01176-5.pdf

Citation 02

https://www.sciencedirect.com/science/article/pii/S2452199X24005309

Citation 03

https://www.nature.com/articles/s41467-023-42972-2

Citation 04

https://advanced.onlinelibrary.wiley.com/doi/abs/10.1002/advs.202417605

Citation 05

https://www.nature.com/articles/s41467-025-58805-3

Citation 06

https://onlinelibrary.wiley.com/doi/abs/10.1002/agt2.70086

Citation 07

https://www.sciencedirect.com/science/article/pii/S1385894724042232

Citation 08

https://www.tandfonline.com/doi/abs/10.1080/19490976.2025.2489072

Citation 09

https://link.springer.com/article/10.1186/s40824-023-00377-8

Citation 10

https://link.springer.com/article/10.1186/s12951-024-02735-7

Citation 11

https://www.sciencedirect.com/science/article/pii/S1043661824005012

Citation 12

https://www.sciencedirect.com/science/article/pii/S004896972404508X

Detection Instrument

Fluorescence Microscope

Available Sizes

Curated Selection

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