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One-step TUNEL In Situ Apoptosis Kit (Green, FITC)

Product Specifications

Detection Method

Fluorometric method

Assay Principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

Sample Type

Paraffin section; frozen section; cell slide

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Product Datasheet

https://789.bio/ea/zjbds8

Product MSDS

https://789.bio/eb/T4ivj9

Citation 01

https://www.nature.com/articles/s41588-023-01528-2

Citation 02

https://www.nature.com/articles/s41368-022-00198-w

Citation 03

https://www.sciencedirect.com/science/article/pii/S2095927323006953

Citation 04

https://www.sciencedirect.com/science/article/pii/S2452199X24001488

Citation 05

https://pubs.acs.org/doi/abs/10.1021/acsnano.3c08485

Citation 06

https://www.nature.com/articles/s41467-023-41782-w

Citation 07

https://academic.oup.com/nar/advance-article-abstract/doi/10.1093/nar/gkae274/7650606

Citation 08

https://www.nature.com/articles/s41467-024-44687-4

Citation 09

https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202302527

Citation 10

https://www.sciencedirect.com/science/article/pii/S1385894723068018

Citation 11

https://pmc.ncbi.nlm.nih.gov/articles/PMC12120566/

Citation 12

https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202406500

Detection Instrument

Fluorescence Microscope

Available Sizes

Curated Selection

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