CD163 antibody

CD163, CD163 antigen, Macrophage-associated antigen, M130, CD163 molecule, Hemoglobin scavenger receptor, MM130; Scavenger receptor cysteine rich type 1 protein M130

Positive Control: A moderate to strong cytoplasmic staining of macrophages should be seen in the interfollicular zones of the tonsil, lamina propria of the appendix and in the Kupffer cells of the liver. Macrophages surrounding the vessels in brain specimens should stain positive, while microglial cells should not show CD163 staining. Negative Control: CD163 staining should not be seen in hepatocytes and in epithelial cells of the appendix and tonsil. Cellular Localization: Membranous and cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.