Melan A CR
Melan A antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA
UniProt
Q16655
Reactivity
Human
Immunogen
Recombinant full-length hMART-1 protein
Clonality
Monoclonal
Clone
MSVA-901M+
Conjugation
Unconjugated
Dilution
1:50-1:100
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Skin: Virtually all melanocytes should show a strong Melan A+ immunostaining including a weak to moderate staining in melanocytic dendrites. Adrenal gland: An at least moderate Melan A+ immunostaining should be seen in adrenocortical cells. Negative Control: Kidney: Melan A immunostaining should be absent in all cells. Adrenal gland: Melan A+ immunostaining should be absent in all medullary cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:75 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG1, kappa
Available Sizes
Frequently Asked Questions
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