Cytokeratin 13 antibody
Cytokeratin 13 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
CK13; Cytokeratin-13; Keratin Type I Cytoskeletal 13; Keratin-13; KRT13; Type I Cytoskeletal 13; WSN2
UniProt
P13646
Reactivity
Human
Immunogen
Esophageal keratins of rabbit origin
Clonality
Monoclonal
Clone
MSVA-613M
Conjugation
Unconjugated
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Tonsil: All squamous epithelial cells of the surface squamous epithelium (except the basal layer) and a fraction of squamous cells of the crypts should show strong KRT13 staining. Negative Control: Tonsil: All lymphocytes and blood vessels must not show any KRT13 staining. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG1, kappa
Available Sizes
Frequently Asked Questions
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