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Annexin V-APC/7-AAD Apoptosis Kit

Product Specifications

Detection Method

Fluorometric method; Flow cytometry

Assay Principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

Sample Type

Cell samples

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2-8°C for 12 months with shading light.

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202306348

Citation 02

https://advanced.onlinelibrary.wiley.com/doi/abs/10.1002/advs.202411020

Citation 03

https://advanced.onlinelibrary.wiley.com/doi/abs/10.1002/advs.202506529

Citation 04

https://link.springer.com/article/10.1186/s12951-024-02781-1

Citation 05

https://www.sciencedirect.com/science/article/pii/S0141813025060349

Citation 06

https://www.nature.com/articles/s41419-025-07713-x

Citation 07

https://spj.science.org/doi/abs/10.34133/bmr.0194

Citation 08

https://www.sciencedirect.com/science/article/pii/S2667005424000875

Citation 09

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9166320/

Citation 10

https://www.hindawi.com/journals/omcl/2023/4952857/

Citation 11

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10548240/

Citation 12

https://link.springer.com/article/10.1186/s12967-023-04403-0

Detection Instrument

Flow Cytometer

Available Sizes

Frequently Asked Questions

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