CD31 antibody
CD31 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA
UniProt
P16284
Reactivity
Human
Immunogen
Recombinant fragment (around aa 625-738) of human CD31 protein (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-031M
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Liver: At least a weak to moderate staining of the majority of the hepatic sinusoidal endothelial cells should be seen. Negative Control: Liver: Hepatocytes should not show any CD31 staining. Cellular Localization: Cell surface and cytoplasm Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG1, kappa
Available Sizes
Frequently Asked Questions
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