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Annexin V-APC/PI Apoptosis Kit

Product Specifications

Detection Method

Fluorometric method; Flow cytometry

Assay Principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

Sample Type

Cell samples

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2-8°C for 12 months with shading light.

Citation 01

https://www.sciencedirect.com/science/article/pii/S1368764623000882

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202202914

Citation 03

https://www.nature.com/articles/s41467-022-30581-4

Citation 04

https://www.nature.com/articles/s41467-024-46190-2

Citation 05

https://www.sciencedirect.com/science/article/pii/S0304389423015261

Citation 06

https://www.sciencedirect.com/science/article/pii/S2213231723004111

Citation 07

https://jeccr.biomedcentral.com/articles/10.1186/s13046-024-03243-0

Citation 08

https://www.sciencedirect.com/science/article/pii/S0168365925000963

Citation 09

https://www.sciencedirect.com/science/article/pii/S104366182200175X

Citation 10

https://onlinelibrary.wiley.com/doi/abs/10.1002/VIW.20230053

Citation 11

https://www.nature.com/articles/s41419-024-07072-z

Citation 12

https://www.nature.com/articles/s41419-025-07502-6

Detection Instrument

Flow Cytometer

Available Sizes

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