Annexin V-PE/7-AAD Apoptosis Kit

Product Specifications

Detection Method

Fluorometric method; Flow cytometry

Assay Principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

Sample Type

Cell samples

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2-8°C for 12 months with shading light.

Citation 01

https://www.sciencedirect.com/science/article/pii/S0141813024082667

Citation 02

https://www.mdpi.com/1422-0067/23/21/13084

Citation 03

https://www.mdpi.com/1999-4923/15/4/1282

Citation 04

https://pubs.acs.org/doi/abs/10.1021/acs.jafc.1c05585

Citation 05

https://www.sciencedirect.com/science/article/pii/S1773224724007901

Citation 06

https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.70006

Citation 07

https://link.springer.com/article/10.1007/s10753-024-02235-8

Citation 08

https://www.nature.com/articles/s41598-022-21601-w

Citation 09

https://jlb.onlinelibrary.wiley.com/doi/abs/10.1002/JLB.3A0121-067RRR

Citation 10

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7585109/

Citation 11

https://www.sciencedirect.com/science/article/pii/S0161589023002341

Citation 12

https://www.nature.com/articles/s41598-024-75874-4

Detection Instrument

Flow Cytometer

Available Sizes

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