PSAP antibody

5′-nucleotidase (5′-NT) ; Acid phosphatase prostate; ACP3; Ecto-5′-nucleotidase; Prostatic acid phosphatase (PAP) ; Prostatic acid phosphatase; Thiamine monophosphatase (TMPase)

Positive Control: Prostate: A strong PSAP immunostaining should, be seen in all glandular cells. Negative Control: Appendix: All epithelial and non-epithelial structures must not show any PSAP immunostaining. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.