MUC1 / EMA antibody

Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma- associated mucin Episialin, Epithelial Membrane Antigen (EMA), H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1- alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen

Positive Control: Kidney: Distal tubuli and collecting ducts must show a strong cytoplasmic MUC1 staining while the staining should be at least moderate in the parietal and visceral sheets of the Bowman’s capsule and largely absent in proximal tubuli. Negative Control: Colon: MUC1 staining should be very weak or absent in colonic epithelium. Cellular Localization: Cytoplasmic & Cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.