S100P antibody

S100P antibody validated for immunohistochemistry on 76 different Normal Tissues

Product Specifications

Product Name Alternative

Migration inducing gene 9 (MIG9) ; S100E; S100 calcium binding protein P

UniProt

P25815

Reactivity

Human

Immunogen

Recombinant fragment corresponding to C-terminal of human S100P protein

Clonality

Monoclonal

Clone

MSVA-480R

Conjugation

Unconjugated

Field of Research

Signal Transduction

Dilution

1:100-1:200

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Urinary bladder: A strong S100P immunostaining should be seen in the urothelium but not in the stroma or muscular cells. Negative Control: Kidney: S100P immunostaining should be completely absent. Cellular Localization: Nucleus and cytoplasm Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.