PTH antibody

PTH antibody validated for immunohistochemistry on 76 different Normal Tissues

Product Specifications

Product Name Alternative

HPTH; Parathormone; Parathyrin; Parathyroid hormone 1 (PTH1) ; Parathyroid hormone (PTH)

UniProt

P01270

Reactivity

Human

Immunogen

A synthetic peptide around aa 1-34 of human mature-PTH-polypeptide (exact sequence is proprietary)

Clonality

Monoclonal

Clone

MSVA-525R

Conjugation

Unconjugated

Dilution

1:100-1:200

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Pituitary gland: A strong PTH immunostaining should be seen in all epithelial cells. Negative Control: Kidney: PTH immunostaining should be absent in all cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.