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SATB2 antibody

SATB2 antibody validated for immunohistochemistry on 76 different Normal Tissues

Product Specifications

Product Name Alternative

DNA-binding protein SATB2; GLSS; SATB homeobox 2; Special AT-rich sequence-binding protein 2

UniProt

Q9UPW6

Reactivity

Human

Immunogen

Synthetic peptide corresponding to residues within aa200-300 corresponding to human SATB2

Clonality

Monoclonal

Clone

MSVA-702R

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Dilution

1:100-1:200

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Colon: A strong nuclear staining should be seen in virtually all columnar epithelial cells. Negative Control: Colon: SATB2 staining should be absent in stromal and smooth muscle cells. Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Tested Applications

IHC

Host or Source

Rabbit

Isotype

IgG

Available Sizes

Frequently Asked Questions

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