SATB2 antibody
SATB2 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
DNA-binding protein SATB2; GLSS; SATB homeobox 2; Special AT-rich sequence-binding protein 2
UniProt
Q9UPW6
Reactivity
Human
Immunogen
Synthetic peptide corresponding to residues within aa200-300 corresponding to human SATB2
Clonality
Monoclonal
Clone
MSVA-702R
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Colon: A strong nuclear staining should be seen in virtually all columnar epithelial cells. Negative Control: Colon: SATB2 staining should be absent in stromal and smooth muscle cells. Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG
Available Sizes
Frequently Asked Questions
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