Histone H3 / H3-3A antibody

Positive Control: Lymph node: A strong nuclear Histone H3 staining should be seen in all cells while strongest staining intensity occurs in cells of germinal centres. Negative Control: Colon, Kidney: Histone H3 staining should be seen in nuclei only. Cytoplasmic staining should not be seen. Cellular Localization: Intracelullar Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.