Histone H3 / H3-3A antibody
Histone H3 / H3-3A antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
H3.3 Histone A, H3.3A, H3F3A, H3F3, H3 Histone Family Member 3A, H3 Histone, Family 3A, Histone H3.3, BRYLIB1, H3- 3B, H3.3B, H3F3B
UniProt
P84243
Reactivity
Human
Immunogen
Recombinant human H3.3 histone A fragment
Clonality
Monoclonal
Clone
MSVA-903M
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Lymph node: A strong nuclear Histone H3 staining should be seen in all cells while strongest staining intensity occurs in cells of germinal centres. Negative Control: Colon, Kidney: Histone H3 staining should be seen in nuclei only. Cytoplasmic staining should not be seen. Cellular Localization: Intracelullar Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG
Available Sizes
Frequently Asked Questions
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