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ACE2 antibody

ACE2 antibody validated for immunohistochemistry on 76 different Normal Tissues

Product Specifications

Product Name Alternative

Angiotensin I converting enzyme 2

UniProt

Q9BYF1

Reactivity

Human

Immunogen

Recombinant fragment (around aa705-805) of human ACE2 (exact sequence is proprietary)

Clonality

Monoclonal

Clone

MSVA-919R

Conjugation

Unconjugated

Dilution

1:100-1:200

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Kidney: Cells of proximal tubuli should show a moderate to strong ACE2 immunostaining with accentuation of the staining at the apical membrane. Negative Control: Kidney: Cells of distal tubuli and glomeruli should not show ACE2 immunostaining. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Tested Applications

IHC

Host or Source

Rabbit

Isotype

IgG

Available Sizes

Frequently Asked Questions

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