ACE2 antibody
ACE2 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
Angiotensin I converting enzyme 2
UniProt
Q9BYF1
Reactivity
Human
Immunogen
Recombinant fragment (around aa705-805) of human ACE2 (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-919R
Conjugation
Unconjugated
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Kidney: Cells of proximal tubuli should show a moderate to strong ACE2 immunostaining with accentuation of the staining at the apical membrane. Negative Control: Kidney: Cells of distal tubuli and glomeruli should not show ACE2 immunostaining. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG
Available Sizes
Frequently Asked Questions
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