Myelin Basic Protein antibody

GDB; Golli MBP; myelin basic protein; Hemopoietic MBP; HMBPR; HUGO; MLD; Myelin A1 Protein, basic; Myelin Deficient; Myelin membrane encephalitogenic protein; SHI; Shiverer; SP

Positive Control: Brain: A fibrillar staining pattern should be seen. Negative Control: Colon: MBP staining should be completely absent. Cellular Localization: Cell surface and cytoplasm. Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.