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Myelin Basic Protein antibody

Myelin Basic Protein antibody validated for immunohistochemistry on 76 different Normal Tissues

Product Specifications

Product Name Alternative

GDB; Golli MBP; myelin basic protein; Hemopoietic MBP; HMBPR; HUGO; MLD; Myelin A1 Protein, basic; Myelin Deficient; Myelin membrane encephalitogenic protein; SHI; Shiverer; SP

UniProt

P02686

Reactivity

Human

Immunogen

Recombinant fragment (around aa 150-250) of human MBP (exact sequence is proprietary)

Clonality

Monoclonal

Clone

MSVA-390R

Conjugation

Unconjugated

Field of Research

Neuroscience

Dilution

1:100-1:200

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Brain: A fibrillar staining pattern should be seen. Negative Control: Colon: MBP staining should be completely absent. Cellular Localization: Cell surface and cytoplasm. Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Tested Applications

IHC

Host or Source

Rabbit

Isotype

IgG

Available Sizes

Frequently Asked Questions

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