Myelin Basic Protein antibody
Myelin Basic Protein antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
GDB; Golli MBP; myelin basic protein; Hemopoietic MBP; HMBPR; HUGO; MLD; Myelin A1 Protein, basic; Myelin Deficient; Myelin membrane encephalitogenic protein; SHI; Shiverer; SP
UniProt
P02686
Reactivity
Human
Immunogen
Recombinant fragment (around aa 150-250) of human MBP (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-390R
Conjugation
Unconjugated
Field of Research
Neuroscience
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Brain: A fibrillar staining pattern should be seen. Negative Control: Colon: MBP staining should be completely absent. Cellular Localization: Cell surface and cytoplasm. Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG
Available Sizes
Frequently Asked Questions
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