GP2 antibody
GP2 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
Glycoprotein 2 (zymogen granule membrane) ; GP2; Pancreatic zymogen granule membrane associated protein GP2; Pancreatic zymogen granule membrane protein GP-2; ZAP75
UniProt
P55259
Reactivity
Human
Immunogen
Recombinant fragment of human GP2 protein (around aa 35-179) (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-475M
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Pancreas: Acinar cells should show a strong immunostaining with apical predominance. Duodenum: Glandular cells of Brunner glands should show a weak to moderate GP2 positivity. Negative Control: Colon: GP2 staining should be completely absent in glandular and stromal cells. Cellular Localization: Cytoplasmic and cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2c, kappa
Available Sizes
Frequently Asked Questions
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