ZAP-70 antibody

Positive Control: Tonsil: A large fraction of the lymphocytes in the interfollicular areas and few lymphocytes in the germinal centres should show a strong ZAP70 immunostaining. Negative Control: Tonsil: ZAP70 immunostaining should be absent in epithelial tissues and the majority of cells in germinal centres. Cellular Localization: Cell Surface and Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.