TACSTD2 / Trop-2 antibody
TACSTD2 / Trop-2 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
Cell surface glycoprotein Trop-2; Membrane Component Chromosome 1, Surface Marker 1 (M1S1) ; Pancreatic Carcinoma Marker Protein GA733-1; TROP2; Tumor-Associated Calcium Signal Transducer 2 (TACSTD2)
UniProt
P09758
Reactivity
Human
Immunogen
Recombinant fragment of human TACSTD2 protein (around aa 31-274) (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-733R
Conjugation
Unconjugated
Field of Research
Cancer Biology; Musculoskeletal & Connective Tissue Research
Purification
Purified from Bioreactor Concentrate by Protein A/G.
Concentration
200ug/ml
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Liver: A strong membranous Trop-2 immunostaining should be seen in bile ducts. Negative Control: Liver: Hepatocytes should not show any Trop-2 immunostaining. Cellular Localization: Cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Preservative
Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available without rAlbumin
Isotype
IgG
Available Sizes
Frequently Asked Questions
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