CELA3B antibody
CELA3B antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
Chymotrypsin like elastase family member 3B (CELA3B) ; ELA3B; Elastase IIIB; Protease E
UniProt
P08861
Reactivity
Human
Immunogen
Recombinant human CELA3B protein fragment (around aa 82-238) (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-410M
Conjugation
Unconjugated
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Pancreas: Acinar cells should show a strong CELA3B immunostaining with apical predominance. Negative Control: Kidney: CELA3B staining should be absent in all epithelial cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG1, kappa
Available Sizes
Frequently Asked Questions
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