S100A12 antibody
S100A12 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
CAAF1, CAGC, CGRP, ENRAGE, MRP6, p6
UniProt
P80511
Reactivity
Human
Clonality
Monoclonal
Clone
MSVA-812M
Conjugation
Unconjugated
Field of Research
Signal Transduction
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Spleen: A significant number of inflammatory cells should show a strong S100A12 immunostaining while the majority of cells remain completely negative. Negative Control: Colon: All epithelial and muscular cells must not show any S100A12 immunostaining. Few scattered S100A12 positive inflammatory cells can occur (mostly within blood vessels) Cellular Localization: Intracellular, Secreted Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG1, kappa
Available Sizes
Frequently Asked Questions
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