Factor XIII alpha antibody
Factor XIII alpha antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
Coagulation factor XIII A chain; Coagulation factor XIII A1 polypeptide; F13A; F13a1; Fibrin stabilizing factor, A subunit; Fibrinoligase; FSF, A subunit; Protein-glutamine gamma-glutamyltransferase A chain; TGase; Transglutaminase. Plasma
UniProt
P00488
Reactivity
Human
Immunogen
Recombinant fragment of human Factor XIIIa protein (aa46-181) (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-813R
Conjugation
Unconjugated
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Colon: A fraction of monocytic cells in the mucosa should show a strong cytoplasmic factor XIIIa immunostaining. Negative Control: Colon: Factor XIIIa immunostaining should be absent in epithelial, endothelial and smooth muscle cells. Cellular Localization: Cytoplasmic and Secreted Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG
Available Sizes
Frequently Asked Questions
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