Factor XIII alpha antibody

Coagulation factor XIII A chain; Coagulation factor XIII A1 polypeptide; F13A; F13a1; Fibrin stabilizing factor, A subunit; Fibrinoligase; FSF, A subunit; Protein-glutamine gamma-glutamyltransferase A chain; TGase; Transglutaminase. Plasma

Positive Control: Colon: A fraction of monocytic cells in the mucosa should show a strong cytoplasmic factor XIIIa immunostaining. Negative Control: Colon: Factor XIIIa immunostaining should be absent in epithelial, endothelial and smooth muscle cells. Cellular Localization: Cytoplasmic and Secreted Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.