P63 antibody

Amplified in squamous cell carcinoma (AIS) ; Chronic ulcerative stomatitis protein (CUSP) ; EEC3; Keratinocyte transcription factor KET; LMS; NBP; p40; P51/P63; p53 like transcription factor; p53-related protein p63; RHS; SHFM4; TAp63alpha; TP53CP; TP53L; TP63; TP73; TP73L; Transformation-related protein 63; Trp53rp1; Trp6;3; Tumor protein 63; Tumor protein p53-like; tumor protein p73-like

Positive Control: Tonsil: Virtually all squamous epithelial cells must show a moderate to strong, nuclear staining, while few scattered lymphocytes and endothelial cells must show a at least a weak staining. Negative Control: Tonsil: The vast majority of lymphocytes should be p63 negative. Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.