Periostin antibody
Periostin antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
Fasciclin-I like; Osteoblast specific factor 2 (OSF2) ; PDLPOSTN; Periodontal ligament specific periostin; Periostin isoform thy2 / th4 / thy6 / thy8; Periostin osteoblast specific factor; PN; POSTN
UniProt
Q15063
Reactivity
Human
Immunogen
Recombinant fragment (around aa193-326) of human POSTN protein (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-649M
Conjugation
Unconjugated
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Skin: A strong periostin immunostaining should be seen in the skin, forming a subepithelial band of variable thickness. Negative Control: Skin: Periostin immunostaining should be lacking in epithelial cells. Cellular Localization: Extracellular space Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2b, kappa
Available Sizes
Frequently Asked Questions
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