Occludin antibody

Occludin antibody validated for immunohistochemistry on 76 different Normal Tissues

Product Specifications

Product Name Alternative

BLCPMG; Occludin; OCLN; Phosphatase 1 regulatory subunit 115; PPP1R115; PTORCH1; Tight junction protein occludin

UniProt

Q16625

Reactivity

Human

Immunogen

Ecombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)

Clonality

Monoclonal

Clone

MSVA-415M

Conjugation

Unconjugated

Dilution

1:100-1:200

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes. Negative Control: Colon: All non-epithelial and non-endothelial cells must not show any occludin staining. Cellular Localization: Cell Surface and Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.