Uroplakin 1A antibody
Uroplakin 1A antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
UP1A; UPIA; UPKA; TSPAN21
UniProt
O00322
Reactivity
Human
Immunogen
Recombinant fragment (around aa 114-173) of human Uroplakin 1A (UPK1A) protein (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-735M
Conjugation
Unconjugated
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Urinary bladder: A strong membranous and cytoplasmic Upk1a immunostaining should be seen in the urothelium (the staining can be limited to the top 1/3 or 2/3 of the urothelium) . Negative Control: Colon: Upk1a immunostaining should be absent in all cells of the colon mucosa. Cellular Localization: Cell surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2b, kappa
Available Sizes
Frequently Asked Questions
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