GLUT1 antibody
GLUT1 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
Erythrocyte/hepatoma glucose transporter; Glucose transporter type-1; GLUT1; GLUT1DS; GLUTB; GT1; GTG1; Gtg3; HepG2 glucose transporter; PED; RATGTG1; Solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1)
UniProt
P11166
Reactivity
Human
Immunogen
Recombinant fragment of human GLUT1 protein (around aa 203-305) (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-401R
Conjugation
Unconjugated
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Brain: A strong GLUT1 staining of endothelial cells should be seen. Negative Control: Brain: GLUT1 staining should be absent in all cells/structures except blood vessels. Cellular Localization: Cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG
Available Sizes
Frequently Asked Questions
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