Glutamine Synthetase antibody
Glutamine Synthetase antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
Cell proliferation-inducing protein 59; GLUL; Glutamate ammonia ligase; GS; PIG43; PIG59; Glutamate decarboxylase; glutamine synthase; Glutamine synthetase; glutamine synthetase I; Proliferation inducing protein 43; GLNA; GLNS
UniProt
P15104
Reactivity
Human
Immunogen
Recombinant fragment (around aa 50-250) of human GLUL protein (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-750M
Conjugation
Unconjugated
Field of Research
Pharmacology & Drug Discovery
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Liver: A strong GS staining should be seen of centrilobular hepatocytes while staining is weak to moderate in Kupffer cells. Negative Control: Liver: Periportal hepatocytes must be GS negative in the normal liver. Cellular Localization: Cytoplasm. Mitochondrion Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2c, kappa
Available Sizes
Frequently Asked Questions
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