Estrogen Receptor antibody

Positive Control: Uterine cervix: virtually all squamous and endocervical cells must show a moderate to strong nuclear staining. Tonsil: Scattered follicular dendritic cells in germinal centers and some squamous epithelial cells in tonsil crypts must show an at least weak nuclear ER immunostaining. Negative Control: Tonsil: B-cells in mantle zones and within germinal centers must be negative. Cellular Localization: Nucleus Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.