Helicobacter Pylori antibody
Helicobacter Pylori antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
H. pylori
Reactivity
Human
Immunogen
Recombinant Helicobacter pylori Catalase protein fragment (around aa323-445) (Exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-466M
Conjugation
Unconjugated
Field of Research
Infectious Disease & Virology
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Gastric tissue with known helicobacter pylori infection should show a distinct staining of bacteria on the surface and/or within glands. Negative Control: Gastric tissue: Normal epithelial and stromal cells should not stain. Cellular Localization: Cell surface. Cytoplasm. Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2b, kappa
Available Sizes
Frequently Asked Questions
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