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XRCC5 / Ku-80

XRCC5 / Ku-80 antibody validated for immunohistochemistry on 76 different Normal Tissues

Product Specifications

Product Name Alternative

ATP-dependent DNA helicase II 80kDa subunit; CTC box-binding factor 85kDa subunit (CTC85) ; CTCBF; DNA repair protein XRCC5; KARP1; Ku autoantigen 80kDa; Ku80; Ku86; KUB2; Lupus Ku autoantigen protein p86; Nuclear factor IV (NFIV) ; Thyroid-lupus autoantigen; TLAA; X-ray repair cross-complementing protein 5 (XRCC5)

UniProt

P13010

Reactivity

Human

Clonality

Monoclonal

Clone

MSVA-880M

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Dilution

1:100-1:200

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Tonsil: A strong staining should be seen in the nuclei of all cells. Negative Control: A tumor or a cell line with documented loss of Ku80 expression. Cellular Localization: Nucleus. Nucleoplasm. Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Tested Applications

IHC

Host or Source

Mouse

Isotype

IgG1 + IgG2b, kappa

Available Sizes

Frequently Asked Questions

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