PSA
PSA antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
Antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin
UniProt
P07288
Reactivity
Human
Immunogen
Recombinant fragment (around aa150-250) of human KLK3 protein (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-603R
Conjugation
Unconjugated
Field of Research
Cancer Biology
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Prostate: The epithelial cells of the prostate glands must show a strong cytoplasmic staining. Due to leakage of the antigen in the vicinity of the prostate glands, adjacent stroma cells may display a weak to moderate staining reaction. Negative Control: Colon: PSA immunostaining should be absent in all cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG
Available Sizes
Frequently Asked Questions
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