MTAP antibody

MTAP antibody validated for immunohistochemistry on 76 different Normal Tissues

Product Specifications

Product Name Alternative

BDMF; DMSFH; DMSMFH; Epididymis luminal protein 249; HEL249; LGMBF; MeSAdo phosphorylase; Methylthioadenosine phosphorylase; MSAP; MTA phosphorylase; MTAPase; S- methyl-5”-thioadenosine phosphorylase

UniProt

Q13126

Reactivity

Human

Immunogen

Recombinant human MTAP protein fragment (aa95-197) (exact sequence is proprietary)

Clonality

Monoclonal

Clone

MSVA-741R

Conjugation

Unconjugated

Field of Research

Cancer Biology

Dilution

1:50-1:100

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Ovary: At least a moderate, nuclear and/or cytoplasmic MTAP staining should be seen in ovarian stroma cells. Negative Control: Bladder cancer with homozygous 9p deletion: MTAP staining should be absent in cells from urothelial tumors with homozygous 9p deletion. Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.