PAX6 antibody
PAX6 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
AN2; Aniridia type II protein; Oculorhombin; Paired box gene 6 (aniridia keratitis) ; Paired box homeotic gene 6; Paired box protein Pax-6; Sey; WAGR
UniProt
P26367
Reactivity
Human
Immunogen
Recombinant human PAX6 fragment
Clonality
Monoclonal
Clone
MSVA-706M
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Pancreas: A strong PAX6 positivity should be seen in the cells of islets of Langerhans. Negative Control: Tonsil: PAX6 immunostaining should be absent in all cells. Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG1, kappa
Available Sizes
Frequently Asked Questions
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