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PAX6 antibody

PAX6 antibody validated for immunohistochemistry on 76 different Normal Tissues

Product Specifications

Product Name Alternative

AN2; Aniridia type II protein; Oculorhombin; Paired box gene 6 (aniridia keratitis) ; Paired box homeotic gene 6; Paired box protein Pax-6; Sey; WAGR

UniProt

P26367

Reactivity

Human

Immunogen

Recombinant human PAX6 fragment

Clonality

Monoclonal

Clone

MSVA-706M

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Dilution

1:100-1:200

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Pancreas: A strong PAX6 positivity should be seen in the cells of islets of Langerhans. Negative Control: Tonsil: PAX6 immunostaining should be absent in all cells. Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Tested Applications

IHC

Host or Source

Mouse

Isotype

IgG1, kappa

Available Sizes

Frequently Asked Questions

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