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Biotinylated Human BLAME / SLAMF8 Protein, His, Avitag™

BLAME (B-lymphocyte activator macrophage expressed) is also known as SLAM family member 8 (SLAMF8), CD353. BLAME is a cell surface receptor that is expressed upon activation of macrophages (MΦs) by IFN-γ or bacteria, is a negative regulator of ROS in response to Gram+ and Gram- bacteria. May play a role in B-lineage commitment and/or modulation of signaling through the B-cell receptor. SLAMF8 is a costimulatory molecule that affects the activation of macrophages in inflammation and in immunosuppression and inflammation response to glioma cells could aid immunotherapy for glioma.

Product Specifications

Background

BLAME (B-lymphocyte activator macrophage expressed) is also known as SLAM family member 8 (SLAMF8), CD353. BLAME is a cell surface receptor that is expressed upon activation of macrophages (MΦs) by IFN-γ or bacteria, is a negative regulator of ROS in response to Gram+ and Gram- bacteria. May play a role in B-lineage commitment and/or modulation of signaling through the B-cell receptor. SLAMF8 is a costimulatory molecule that affects the activation of macrophages in inflammation and in immunosuppression and inflammation response to glioma cells could aid immunotherapy for glioma.

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 27.1 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q9P0V8-1

Expression Region

Ala 23 - Asp 233

Host

HEK293

Target

BLAME

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

85%

Bioactivity

Immobilized Human BLAME, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human BLAME, His,Avitag (Cat. No. BLE-H82E4) with a linear range of 1-31 ng/mL (QC tested).

Format

Powder

Buffer

50 mM Sodium Citrate, 150 mM NaCl, pH5.5

Additives

Trehalose

Molecular Weight

27.1 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

200ug*1

Host or Source

HEK293

Species

Human

Protein ID

Q9P0V8-1

Preservative

Trehalose

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